chelex extraction protocol
Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including Mg 2 which is an essential cofactor for DNases. The aim of this method was to provide a protocol for DNA extraction which was less expensive than silica-based or magnetic separation kits and less. Measures Of Chelex Dna Extraction Efficiency Download Table The wet capacity of Chelex resin is 040 meqml. . Make 20 and 5 wtvol stock solutions in sterile distilled water to a final volume of 100-300 ml. Note on handling Chelex solutions. Using the Chelex protocol of Musapa et al. This can be achieved by gentle mixing with a stir bar in a beaker. Add 200µl to 500µl of Chelex solution and sample to eppie tube. Add 1 uL of proteinase K solution 20mgmL to each tube. We morphologically identified 72 Anopheles gambiae sl. Label the box with your last name the date and US. Walsh PS Metzger DA and